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AccediRna Interference
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Rna Interference
Da Gex Gio 13 Gen - 21:48
La RNA interference (dall'inglese interferenza dell'RNA, abbreviata comunemente come RNAi) è un meccanismo mediante il quale alcuni frammenti di RNA a doppio filamento sono in grado di interferire (e spegnere) l'espressione genica.
La RNAi è distinta da altri fenomeni di silenziamento genetico ed è stata osservata essere in grado di diffondere da cellula a cellula e di essere ereditabile.
Si sta cercando di applicare questa tecnica alla lotta all'Hiv.
In pratica si isolano molti shRNAs per bloccare l'espressione genica dell'hiv.
[Devi essere iscritto e connesso per vedere questa immagine]
In figura si vede come funziona la tecnica dell' RNAi: somministrando dall'esterno siRNA che si associ a RISC, è possibile degradare specifici mRNA.
[Devi essere iscritto e connesso per vedere questo link]
Multiple shRNA combinations for near-complete coverage of all HIV-1 strains
Combinatorial RNA interference (co-RNAi) approaches are needed to account for viral variability in treating HIV-1 with RNAi, as single short hairpin RNAs (shRNA) are rapidly rendered ineffective by resistant strains. Current work suggests that 4 simultaneously expressed shRNAs may prevent the emergence of resistant strains.
Results: In this study we assembled combinations of highly-conserved shRNAs to target as many HIV-1 strains as possible.
We analyzed intersecting conservations of 10 shRNAs to find combinations with 4+ matching the maximum number of strains using 1220+ HIV-1 sequences from the Los Alamos National Laboratory (LANL). We built 26 combinations of 2 to 7 shRNAs with up to 87 % coverage for all known strains and 100 % coverage of clade B subtypes, and characterized their intrinsic suppressive activities in transient expression assays.
We found that all combinations had high combined suppressive activities, though there were also large changes in the individual activities of the component shRNAs in our multiple expression cassette configurations.
Conclusion: By considering the intersecting conservations of shRNA combinations we have shown that it is possible to assemble combinations of 6 and 7 highly active, highly conserved shRNAs such that there is always at least 4 shRNAs within each combination covering all currently known variants of entire HIV-1 subtypes. By extension, it may be possible to combine several combinations for complete global coverage of HIV-1 variants.
Author: Glen McintyreJennifer GronemanYi-Hsin YuAnna TranTanya Applegate
Credits/Source: AIDS Research and Therapy 2011, 8:1
La RNAi è distinta da altri fenomeni di silenziamento genetico ed è stata osservata essere in grado di diffondere da cellula a cellula e di essere ereditabile.
Si sta cercando di applicare questa tecnica alla lotta all'Hiv.
In pratica si isolano molti shRNAs per bloccare l'espressione genica dell'hiv.
[Devi essere iscritto e connesso per vedere questa immagine]
In figura si vede come funziona la tecnica dell' RNAi: somministrando dall'esterno siRNA che si associ a RISC, è possibile degradare specifici mRNA.
[Devi essere iscritto e connesso per vedere questo link]
Multiple shRNA combinations for near-complete coverage of all HIV-1 strains
Combinatorial RNA interference (co-RNAi) approaches are needed to account for viral variability in treating HIV-1 with RNAi, as single short hairpin RNAs (shRNA) are rapidly rendered ineffective by resistant strains. Current work suggests that 4 simultaneously expressed shRNAs may prevent the emergence of resistant strains.
Results: In this study we assembled combinations of highly-conserved shRNAs to target as many HIV-1 strains as possible.
We analyzed intersecting conservations of 10 shRNAs to find combinations with 4+ matching the maximum number of strains using 1220+ HIV-1 sequences from the Los Alamos National Laboratory (LANL). We built 26 combinations of 2 to 7 shRNAs with up to 87 % coverage for all known strains and 100 % coverage of clade B subtypes, and characterized their intrinsic suppressive activities in transient expression assays.
We found that all combinations had high combined suppressive activities, though there were also large changes in the individual activities of the component shRNAs in our multiple expression cassette configurations.
Conclusion: By considering the intersecting conservations of shRNA combinations we have shown that it is possible to assemble combinations of 6 and 7 highly active, highly conserved shRNAs such that there is always at least 4 shRNAs within each combination covering all currently known variants of entire HIV-1 subtypes. By extension, it may be possible to combine several combinations for complete global coverage of HIV-1 variants.
Author: Glen McintyreJennifer GronemanYi-Hsin YuAnna TranTanya Applegate
Credits/Source: AIDS Research and Therapy 2011, 8:1
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